Restricted expression of LUZP in neural lineage cells: a study in embryonic stem cells

J Biomed Sci. Nov-Dec 2001;8(6):504-11. doi: 10.1007/BF02256613.

Abstract

A novel protein LUZP with 3 leucine zipper motifs at its amino terminus is predominantly expressed in the adult brain. A modified gene targeting approach was employed in an attempt to establish in vitro and in vivo models in which Luzp is knock-out (KO) for phenotype assessment and a reporter gene lacZ is knock-in (KI) for tracing its expression. We report in this study the molecular cloning of the Luzp gene, its targeting vector construction and Luzp-KO/lacZ-KI embryonic stem (ES) clone selection. Since LUZP is also expressed in ES cells, the possibility of embryonic lethality cannot be excluded when attempting to establish Luzp-null mutant mice. We have therefore examined the development of homozygous Luzp-KO/lacZ-KI clones in nude mice. Tissue types derived from all three embryonic germ layers were observed in teratomas developed in nude mice. In situ X-gal staining further revealed restricted expression of LUZP in neural lineage cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage / genetics*
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Galactosides
  • Gene Targeting
  • Genetic Vectors
  • Hematopoietic Stem Cell Transplantation
  • Immunohistochemistry
  • Indoles
  • Lac Operon / genetics
  • Male
  • Mice
  • Mice, Nude
  • Neurons / chemistry*
  • Neurons / cytology
  • Proteins / genetics
  • Proteins / metabolism*
  • Proteins / physiology
  • Stem Cells / metabolism
  • Teratoma / chemistry
  • Teratoma / pathology
  • Tissue Distribution

Substances

  • DNA-Binding Proteins
  • Galactosides
  • Indoles
  • Luzp1 protein, mouse
  • Proteins
  • 5-bromo-4-chloro-3-indolyl beta-galactoside