Humoral immune response to autologous blood transfusion in hip surgery: whole blood versus packed red cells and plasma

Vox Sang. 2001 Oct;81(3):180-6. doi: 10.1046/j.1423-0410.2001.00106.x.

Abstract

Background and objectives: The immune response to the transfused autologous buffy coat content in whole blood has, to date, not been studied in detail.

Subjects and methods: Patients undergoing hip arthroplasty were studied according to whether they received autologous whole blood (WB) (n = 30), autologous fresh-frozen plasma and buffy coat-poor red cells (RC) (n = 40), or no transfusion (NT) (n = 27). Plasma levels of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and complement SC5b-9 were analysed by enzyme-linked immunosorbent assay (ELISA) 7 days after surgery.

Results: There were no significant between-group differences regarding the time course of TNF-alpha, IL-6 and complement SC5b-9 plasma level changes, the infection rate, or the length of hospital stay.

Conclusion: In comparison to the impact of surgery on cytokine and complement levels, the transfusion of autologous buffy coat is not relevant.

Publication types

  • Clinical Trial
  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibody Formation / drug effects*
  • Arthroplasty, Replacement, Hip / methods*
  • Blood Component Transfusion / adverse effects
  • Blood Component Transfusion / methods
  • Blood Preservation / methods
  • Blood Transfusion, Autologous / adverse effects*
  • Blood Transfusion, Autologous / methods
  • Cohort Studies
  • Complement Membrane Attack Complex
  • Complement System Proteins / analysis
  • Complement System Proteins / immunology
  • Complement System Proteins / pharmacology
  • Cryopreservation / methods
  • Female
  • Glycoproteins / analysis
  • Glycoproteins / immunology
  • Glycoproteins / pharmacology
  • Humans
  • Infections / etiology
  • Inflammation Mediators / analysis
  • Inflammation Mediators / immunology
  • Inflammation Mediators / pharmacology
  • Interleukin-6 / analysis
  • Interleukin-6 / immunology
  • Interleukin-6 / pharmacology
  • Length of Stay
  • Male
  • Middle Aged
  • Time Factors
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Complement Membrane Attack Complex
  • Glycoproteins
  • Inflammation Mediators
  • Interleukin-6
  • SC5b-9 protein complex
  • Tumor Necrosis Factor-alpha
  • Complement System Proteins