Surfactant Protein D Enhances Bacterial Antigen Presentation by Bone Marrow-Derived Dendritic Cells

Am J Physiol Lung Cell Mol Physiol. 2001 Dec;281(6):L1453-63. doi: 10.1152/ajplung.2001.281.6.L1453.

Abstract

Surfactant protein (SP) D functions as a soluble pattern recognition molecule to mediate the clearance of pathogens by phagocytes in the innate immune response. We hypothesize that SP-D may also interact with dendritic cells, the most potent antigen presenting cell, to enhance uptake and presentation of bacterial antigens. Using mouse bone marrow-derived dendritic cells, we show that SP-D binds to immature dendritic cells in a dose-, carbohydrate-, and calcium-dependent manner, whereas SP-D binding to mature dendritic cells is reduced. SP-D also binds to Escherichia coli HB101 and enhances its association with dendritic cells. Additionally, SP-D enhances the antigen presentation of an ovalbumin fusion protein expressed in E. coli HB101 to ovalbumin-specific major histocompatibility complex class II T cell hybridomas. The enhancement of antigen presentation by SP-D is dose dependent and is not shared by other collectin-like proteins tested. These studies demonstrate that SP-D augments antigen presentation by dendritic cells and suggest that innate immune molecules such as SP-D may help initiate an adaptive immune response for the purpose of resolving an infection.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation / drug effects*
  • Antigen Presentation / immunology
  • Antigens, Bacterial / immunology*
  • Antigens, Bacterial / metabolism
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / immunology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Epitopes / genetics
  • Epitopes / immunology
  • Escherichia coli / immunology
  • Gene Expression
  • Glycoproteins / immunology
  • Glycoproteins / metabolism
  • Glycoproteins / pharmacology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Lung / cytology
  • Lung / immunology
  • Mice
  • Mice, Inbred C57BL
  • Ovalbumin / genetics
  • Ovalbumin / immunology
  • Phagocytosis / drug effects
  • Phagocytosis / immunology
  • Protein Binding / immunology
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactants / immunology
  • Pulmonary Surfactants / metabolism
  • Pulmonary Surfactants / pharmacology*

Substances

  • Antigens, Bacterial
  • Epitopes
  • Glycoproteins
  • Pulmonary Surfactant-Associated Protein D
  • Pulmonary Surfactants
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Ovalbumin