Malignant transformation of primary chicken spleen cells by human transcription factor c-Rel

Oncogene. 2001 Oct 25;20(48):7098-103. doi: 10.1038/sj.onc.1204898.


Rel/NF-kappaB transcription factors control a variety of cellular processes, such as cell growth and apoptosis, that are relevant to oncogenesis, and mutations in genes encoding Rel/NF-kappaB transcription factors have been found in several human lymphoid cell cancers. In this study, we have used a sensitive cell outgrowth assay to demonstrate that wild-type human c-Rel can malignantly transform primary chicken spleen cells, and that transformation by c-Rel is accelerated by co-expression of Bc1-2. Full-length mouse c-Rel can also transform chicken spleen cells. These results are the first demonstration of a lymphoid cell malignant transforming ability for mammalian Rel/NF-kappaB transcription factors, and implicate c-Rel as a molecular target for cancer therapeutics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agar
  • Animals
  • Apoptosis / genetics
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic / genetics*
  • Chickens
  • Colony-Forming Units Assay / methods
  • Culture Media
  • Gene Expression Regulation, Neoplastic
  • Genes, bcl-2
  • Genes, rel
  • Humans
  • Mice
  • NF-kappa B / physiology
  • Oncogene Proteins v-rel / physiology
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / physiology
  • Proto-Oncogene Proteins c-rel / genetics
  • Proto-Oncogene Proteins c-rel / physiology*
  • Recombinant Fusion Proteins / physiology
  • Signal Transduction
  • Species Specificity
  • Spleen / cytology*
  • Transcription, Genetic
  • Transfection
  • bcl-X Protein


  • BCL2L1 protein, human
  • Bcl2l1 protein, mouse
  • Culture Media
  • NF-kappa B
  • Oncogene Proteins v-rel
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins c-rel
  • Recombinant Fusion Proteins
  • bcl-X Protein
  • Agar