Reversal of compromised bonding to oxidized etched dentin

J Dent Res. 2001 Oct;80(10):1919-24. doi: 10.1177/00220345010800101101.


The mechanism responsible for hydrogen-peroxide- or sodium-hypochlorite-induced reductions in dentin bond strength is unknown. This in vitro study tested the hypothesis that these oxidizing agents were responsible by attempting to reverse the effect with sodium ascorbate, a reducing agent. Human dentin was treated with these oxidants before or after being acid-etched and with or without post-treatment with sodium ascorbate. They were bonded with either Single Bond or Excite. Hydrogen peroxide reduced the bond strengths of both adhesives, while sodium hypochlorite produced reduction in adhesion of only Single Bond (p < 0.05). Following treatment with sodium ascorbate, reductions in bond strength were reversed. Transmission and scanning electron microscopy showed partial removal of the demineralized collagen matrix only by sodium hypochlorite. The observed compromised bond strengths cannot be attributed to incomplete deproteinization and may be related to changes in the redox potential of the bonding substrates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acid Etching, Dental*
  • Adhesiveness
  • Analysis of Variance
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Ascorbic Acid / chemistry
  • Ascorbic Acid / pharmacology*
  • Bisphenol A-Glycidyl Methacrylate / chemistry
  • Collagen / drug effects
  • Collagen / ultrastructure
  • Dental Bonding*
  • Dentin / drug effects*
  • Dentin / ultrastructure
  • Dentin-Bonding Agents / chemistry
  • Humans
  • Hydrogen Peroxide / chemistry
  • Hydrogen Peroxide / pharmacology*
  • Methacrylates / chemistry
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Oxidants / chemistry
  • Oxidants / pharmacology*
  • Oxidation-Reduction
  • Sodium Hypochlorite / chemistry
  • Sodium Hypochlorite / pharmacology*
  • Statistics as Topic
  • Stress, Mechanical
  • Surface Properties
  • Tensile Strength


  • Antioxidants
  • Dentin-Bonding Agents
  • Excite cement
  • Methacrylates
  • Oxidants
  • single bond
  • Bisphenol A-Glycidyl Methacrylate
  • Collagen
  • Hydrogen Peroxide
  • Sodium Hypochlorite
  • Ascorbic Acid