Striatin, a calmodulin-dependent scaffolding protein, directly binds caveolin-1

FEBS Lett. 2001 Nov 9;508(1):49-52. doi: 10.1016/s0014-5793(01)03020-4.


Caveolins are scaffolding proteins able to collect on caveolae a large number of signalling proteins bearing a caveolin-binding motif. The proteins of the striatin family, striatin, SG2NA, and zinedin, are composed of several conserved, collinearly aligned, protein-protein association domains, among which a putative caveolin-binding domain [Castets et al. (2000) J. Biol. Chem. 275, 19970-19977]. They are associated in part with membranes. These proteins are mainly expressed within neurons and thought to act both as scaffolds and as Ca(2+)-dependent signalling proteins [Bartoli et al. (1999) J. Neurobiol. 40, 234-243]. Here, we show that (1) rat brain striatin, SG2NA and zinedin co-immunoprecipitate with caveolin-1; (2) all are pulled down by glutathione-S-transferase (GST)-caveolin-1; (3) a fragment of recombinant striatin containing the putative caveolin-binding domain binds GST-caveolin-1. Hence, it is likely that the proteins of the striatin family are addressed to membrane microdomains by their binding to caveolin, in accordance with their putative role in membrane trafficking [Baillat et al. (2001) Mol. Biol. Cell 12, 663-673].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoantigens / metabolism
  • Calmodulin / metabolism*
  • Calmodulin-Binding Proteins / metabolism*
  • Caveolin 1
  • Caveolins / immunology
  • Caveolins / metabolism*
  • Cholic Acids / chemistry
  • Detergents / chemistry
  • Membrane Proteins / metabolism*
  • Nerve Tissue Proteins / metabolism*
  • Precipitin Tests
  • Protein Binding
  • Rats
  • Rats, Wistar
  • Recombinant Fusion Proteins / metabolism


  • Autoantigens
  • Calmodulin
  • Calmodulin-Binding Proteins
  • Cav1 protein, rat
  • Caveolin 1
  • Caveolins
  • Cholic Acids
  • Detergents
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • STRN protein, human
  • Strn protein, rat
  • Strn3 protein, rat
  • zinedin
  • 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate