CD5 expression by B lymphocytes and its regulation upon Epstein-Barr virus transformation

Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13850-3. doi: 10.1073/pnas.241509398. Epub 2001 Nov 13.


Dim expression of CD5 on human B lymphocytes has been used to delineate B1 and B2 subsets. Nevertheless, others have suggested that the molecule is an activation marker and does not predicate a subset distinction. We have used enzymatic amplification staining, a technology that enhances the resolution of flow cytometric analysis of cell surface molecules by as much as 100-fold, to determine that essentially all human B cells express CD5. Furthermore, we show that this expression is regulated during Epstein-Barr virus transformation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocytes / classification
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • CD5 Antigens / biosynthesis
  • CD5 Antigens / genetics*
  • Cell Line, Transformed
  • Cells, Cultured
  • Gene Expression*
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Transformation, Genetic


  • CD5 Antigens