Endogenous melanin-concentrating hormone receptor SLC-1 in human melanoma SK-MEL-37 cells

Biochem Biophys Res Commun. 2001 Nov 23;289(1):44-50. doi: 10.1006/bbrc.2001.5926.


Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide that regulates several physiological functions. The orphan G protein-coupled receptors SLC-1 and MCHR2 were recently found to bind MCH with high affinity. We show here that the human melanoma cell line SK-MEL-37 expresses SLC-1 mRNA but not MCHR2 by RT-PCR analysis and immunofluorescence studies. Using Chinese hamster ovary cells and 293 cells overexpressing SLC-1 by cDNA transfection, it was shown that SLC-1 coupled to both G alpha(i)/G alpha(o) and G alpha(q) proteins. In SK-MEL-37 cells, MCH inhibited forskolin-stimulated cyclic AMP accumulation and induced mitogen-activated protein kinase (MAPK) in a pertussis toxin-(PTX)-sensitive manner. The MAPK activity leads to the production of phosphorylated forms of p42/p44 MAPK. However, an increase in the intracellular free Ca(2+) concentration was not elicited by MCH in SK-MEL-37 cells. These results show that SLC-1 is coupled only to PTX-sensitive G alpha(i)/G alpha(o) in SK-MEL-37 cells. This study provides for the first time a skin-derived cellular model to analyze the molecular mechanism of the MCH signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Colforsin / pharmacology
  • Cricetinae
  • Cyclic AMP / metabolism
  • Enzyme Induction / drug effects
  • Heterotrimeric GTP-Binding Proteins / metabolism
  • Humans
  • Hypothalamic Hormones / metabolism*
  • Hypothalamic Hormones / pharmacology
  • Melanins / metabolism*
  • Melanins / pharmacology
  • Melanoma / genetics
  • Melanoma / metabolism*
  • Mitogen-Activated Protein Kinases / biosynthesis
  • Models, Biological
  • Pertussis Toxin
  • Phosphorylation
  • Pituitary Hormones / metabolism*
  • Pituitary Hormones / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Receptors, Pituitary Hormone / genetics
  • Receptors, Pituitary Hormone / metabolism*
  • Signal Transduction
  • Tumor Cells, Cultured
  • Virulence Factors, Bordetella / pharmacology


  • Hypothalamic Hormones
  • Melanins
  • Pituitary Hormones
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Pituitary Hormone
  • Virulence Factors, Bordetella
  • melanin-concentrating hormone receptor
  • Colforsin
  • melanin-concentrating hormone
  • Cyclic AMP
  • Pertussis Toxin
  • Mitogen-Activated Protein Kinases
  • Heterotrimeric GTP-Binding Proteins