Effects of cocaine- and amphetamine-regulated transcript peptide, leptin and orexins on hypothalamic serotonin release

Eur J Pharmacol. 2001 Nov 2;430(2-3):269-72. doi: 10.1016/s0014-2999(01)01380-2.


We have studied the effects of cocaine- and amphetamine-regulated transcript (CART) peptide-(55-102), leptin, orexin-A and orexin-B on basal and depolarization (K(+) 15 mM)-induced serotonin (5-hydroxytryptamine, 5-HT) release from rat hypothalamic neuronal endings (synaptosomes) in vitro. We have found that leptin and CART peptide-(55-102) have no effect on 5-HT release, while orexin-A and orexin-B inhibit depolarization-stimulated serotonin release. We can conclude that leptin and CART peptide-(55-102), which play a physiological role as feeding inhibitors, do not acutely affect 5-HT release from hypothalamic synaptosomes; on the other hand, feeding induced by orexin-A and orexin-B could be partially explained by decreased 5-HT release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / pharmacology
  • Dose-Response Relationship, Drug
  • Hypothalamus / drug effects*
  • Hypothalamus / metabolism
  • Intracellular Signaling Peptides and Proteins*
  • Leptin / pharmacology
  • Male
  • Nerve Tissue Proteins / pharmacology
  • Neuropeptides / pharmacology
  • Orexins
  • Potassium / pharmacology
  • Proteins / pharmacology*
  • Rats
  • Rats, Wistar
  • Serotonin / metabolism*
  • Time Factors
  • Tritium


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Leptin
  • Nerve Tissue Proteins
  • Neuropeptides
  • Orexins
  • Proteins
  • cocaine- and amphetamine-regulated transcript protein
  • Tritium
  • Serotonin
  • Potassium