Optical imaging of neural activity in multiple auditory cortical fields of guinea pigs

Neuroreport. 2001 Oct 29;12(15):3335-9. doi: 10.1097/00001756-200110290-00038.


Neural activity of multiple fields in the auditory cortex of anesthetized guinea pigs in response to pure tones was visualized by optical recording using a voltage-sensitive dye (RH795). Ten auditory fields were identified based on the tonotopic organization and response latency: the core fields consisting of the primary (AI) and secondary (AII) fields and surrounding belt areas consisting of dorso-anterior (DA), dorsal (D), dorso-posterior (DP), posterior (P), ventro-posterior (VP), ventro-medial (VM), ventro-anterior (VA) and ventral (V) fields. Tonotopic organization was observed in all the fields apart from DA, D, DP and V. Spatio-temporal displays suggest that the auditory information spreads from the core fields of AI and AII to belt fields via three distinct (dorsocaudal, caudal and ventrorostral) pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustic Stimulation
  • Action Potentials / physiology
  • Animals
  • Auditory Cortex / anatomy & histology
  • Auditory Cortex / physiology*
  • Brain Mapping / instrumentation
  • Brain Mapping / methods*
  • Electronic Data Processing / instrumentation
  • Electronic Data Processing / methods*
  • Evoked Potentials, Auditory / physiology*
  • Fluorescent Dyes*
  • Guinea Pigs
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Neural Pathways / anatomy & histology
  • Neural Pathways / physiology
  • Neurons / physiology*
  • Reaction Time / physiology
  • Styrenes*


  • Fluorescent Dyes
  • RH 795
  • Styrenes