An mRNA degrading complex in Rhodobacter capsulatus

Nucleic Acids Res. 2001 Nov 15;29(22):4581-8. doi: 10.1093/nar/29.22.4581.

Abstract

An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.

MeSH terms

  • Amino Acid Sequence
  • Antibodies / immunology
  • Cell Fractionation
  • Centrifugation / methods
  • Endoribonucleases / immunology
  • Endoribonucleases / metabolism
  • Macromolecular Substances
  • Molecular Sequence Data
  • Precipitin Tests
  • RNA Helicases / metabolism
  • RNA Processing, Post-Transcriptional
  • RNA, Messenger / metabolism*
  • Rhodobacter capsulatus / chemistry
  • Rhodobacter capsulatus / genetics
  • Rhodobacter capsulatus / metabolism*
  • Sequence Analysis, Protein

Substances

  • Antibodies
  • Macromolecular Substances
  • RNA, Messenger
  • Endoribonucleases
  • ribonuclease E
  • RNA Helicases