Abstract
Expulsion of two gastrointestinal nematode parasites, Nippostrongylus brasiliensis and Trichinella spiralis, is similar in that both require IL-4Ralpha expression, but different in that T cells and mast cells are required for IL-4-induced expulsion of T. spiralis but not N. brasiliensis. To examine the role of IL-4Ralpha signaling in immunity to these parasites, we studied worm expulsion in chimeric mice that selectively expressed IL-4Ralpha on bone marrow-derived or non-bone marrow-derived cells. N. brasiliensis was expelled by mice that expressed IL-4Ralpha only on non-bone marrow-derived cells, but not by mice that expressed IL-4Ralpha only on bone marrow-derived cells. Although T. spiralis expulsion required IL-4Ralpha expression by both bone marrow- and non-bone marrow-derived cells, IL-4 stimulation eliminated the requirement for IL-4Ralpha expression by bone marrow-derived cells. Thus, direct IL-4Ralpha signaling of nonimmune gastrointestinal cells may be generally required to induce worm expulsion, even when mast cell and T cell responses are also required.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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B-Lymphocytes / pathology
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Bone Marrow Cells / immunology*
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Bone Marrow Cells / metabolism
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Bone Marrow Cells / parasitology
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Female
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Gastrointestinal Diseases / immunology*
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Gastrointestinal Diseases / parasitology*
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Gastrointestinal Diseases / prevention & control
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Interleukin-4 / physiology
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Lymphopenia / genetics
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Lymphopenia / immunology
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Lymphopenia / parasitology
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Mast Cells / immunology*
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Mast Cells / metabolism
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Mast Cells / pathology
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Mice, Nude
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Mice, SCID
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Nippostrongylus / immunology*
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Receptors, Interleukin-4 / biosynthesis*
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Receptors, Interleukin-4 / deficiency
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Receptors, Interleukin-4 / genetics
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Strongylida Infections / immunology
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Strongylida Infections / parasitology
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Strongylida Infections / prevention & control
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T-Lymphocytes / immunology*
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T-Lymphocytes / metabolism
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T-Lymphocytes / pathology
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Trichinella spiralis / immunology*
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Trichinellosis / immunology
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Trichinellosis / parasitology
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Trichinellosis / prevention & control
Substances
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Receptors, Interleukin-4
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Interleukin-4