Abstract
Lembehyne A (LB-A), a spongean polyacetylene, induced neuronal cell differentiation in a neuroblastoma cell line, Neuro 2A. The LB-A treatment of Neuro 2A cells predominantly resulted in a morphological change with bipolar neurites. The acetylcholinesterase activity of Neuro 2A was also increased by the treatment of LB-A. Furthermore, the cell cycle of Neuro 2A cells was found to be specifically blocked at the G1 phase by LB-A. The structure-activity relationship study using the LB-A analogues revealed the importance of the terminal 1-yn-3-ol and unsaturated long-chain alkyl moieties for the neuronal differentiation activity of LB-A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholinesterase / metabolism
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Acetylene / analogs & derivatives*
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Acetylene / chemistry*
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Alkynes / chemistry
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Alkynes / metabolism*
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Alkynes / pharmacology
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Animals
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Cell Cycle
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Cell Differentiation
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Cell Line
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Cell Separation
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Cells, Cultured
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Dose-Response Relationship, Drug
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Fatty Alcohols / chemistry
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Fatty Alcohols / metabolism*
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Fatty Alcohols / pharmacology
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Flow Cytometry
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Humans
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Models, Chemical
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Neurons / cytology
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Neurons / metabolism*
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PC12 Cells
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Polymers / chemistry*
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Polyynes
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Porifera
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Rats
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Structure-Activity Relationship
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Time Factors
Substances
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Alkynes
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Fatty Alcohols
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Polymers
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lembehyne A
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Polyynes
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Acetylcholinesterase
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Acetylene