Lembehyne A, a spongean polyacetylene, induces neuronal differentiation in neuroblastoma cell

Biochem Biophys Res Commun. 2001 Nov 30;289(2):558-63. doi: 10.1006/bbrc.2001.6012.

Abstract

Lembehyne A (LB-A), a spongean polyacetylene, induced neuronal cell differentiation in a neuroblastoma cell line, Neuro 2A. The LB-A treatment of Neuro 2A cells predominantly resulted in a morphological change with bipolar neurites. The acetylcholinesterase activity of Neuro 2A was also increased by the treatment of LB-A. Furthermore, the cell cycle of Neuro 2A cells was found to be specifically blocked at the G1 phase by LB-A. The structure-activity relationship study using the LB-A analogues revealed the importance of the terminal 1-yn-3-ol and unsaturated long-chain alkyl moieties for the neuronal differentiation activity of LB-A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / metabolism
  • Acetylene / analogs & derivatives*
  • Acetylene / chemistry*
  • Alkynes / chemistry
  • Alkynes / metabolism*
  • Alkynes / pharmacology
  • Animals
  • Cell Cycle
  • Cell Differentiation
  • Cell Line
  • Cell Separation
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Fatty Alcohols / chemistry
  • Fatty Alcohols / metabolism*
  • Fatty Alcohols / pharmacology
  • Flow Cytometry
  • Humans
  • Models, Chemical
  • Neurons / cytology
  • Neurons / metabolism*
  • PC12 Cells
  • Polymers / chemistry*
  • Polyynes
  • Porifera
  • Rats
  • Structure-Activity Relationship
  • Time Factors

Substances

  • Alkynes
  • Fatty Alcohols
  • Polymers
  • lembehyne A
  • Polyynes
  • Acetylcholinesterase
  • Acetylene