Comprehensive DNA Microarray Analysis of Bacillus Subtilis Two-Component Regulatory Systems

J Bacteriol. 2001 Dec;183(24):7365-70. doi: 10.1128/JB.183.24.7365-7370.2001.

Abstract

It has recently been shown through DNA microarray analysis of Bacillus subtilis two-component regulatory systems (DegS-DegU, ComP-ComA, and PhoR-PhoP) that overproduction of a response regulator of the two-component systems in the background of a deficiency of its cognate sensor kinase affects the regulation of genes, including its target ones. The genome-wide effect on gene expression caused by the overproduction was revealed by DNA microarray analysis. In the present work, we newly analyzed 24 two-component systems by means of this strategy, leaving out 8 systems to which it was unlikely to be applicable. This analysis revealed various target gene candidates for these two-component systems. It is especially notable that interesting interactions appeared to take place between several two-component systems. Moreover, the probable functions of some unknown two-component systems were deduced from the list of their target gene candidates. This work is heuristic but provides valuable information for further study toward a comprehensive understanding of the B. subtilis two-component regulatory systems. The DNA microarray data obtained in this work are available at the KEGG Expression Database website (http://www.genome.ad.jp/kegg/expression).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Base Sequence
  • DNA Primers
  • Down-Regulation
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Histidine Kinase
  • Models, Biological
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Protein Kinases / genetics*
  • Signal Transduction / genetics*
  • Transcription Factors / genetics*
  • Up-Regulation

Substances

  • DNA Primers
  • Transcription Factors
  • Protein Kinases
  • Histidine Kinase