Clamp loader structure predicts the architecture of DNA polymerase III holoenzyme and RFC

Curr Biol. 2001 Nov 13;11(22):R935-46. doi: 10.1016/s0960-9822(01)00559-0.

Abstract

Recent determinations of the crystal structure of the Escherichia coli gamma complex and delta-beta assembly have shed light on the bacterial clamp loading reaction. In this review, we discuss the structures of delta-beta and the gamma(3)deltadelta' complex and its mechanism of action as a clamp loader of the E. coli beta sliding clamp. We also expand upon the implications of the structural findings to the structure and function of the eukaryotic clamp loader, RFC, and the structure of E. coli DNA polymerase III holoenzyme.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Crystallography, X-Ray
  • DNA Polymerase III / chemistry*
  • Escherichia coli / enzymology
  • Eukaryotic Cells
  • Holoenzymes / chemistry
  • Humans
  • Protein Structure, Tertiary

Substances

  • Holoenzymes
  • DNA Polymerase III