Release of norepinephrine from human ovary: coupling to steroidogenic response

Endocrine. 2001 Jul;15(2):187-92. doi: 10.1385/ENDO:15:2:187.

Abstract

We investigated the possibility that norepinephrine from the human ovary is released after nerve stimulation and that this neurotransmitter is coupled to a steroidogenic response. Biologically significant levels of both norepinephrine and dopamine were found in human ovarian biopsies. [3H]norepinephrine incorporated in vitro was readily released by electrical stimulation in a Ca2+-dependent process. Ovarian membrane preparations exhibited specific binding sites for the beta-adrenergic antagonist [3H]dihydroalprenolol. Displacement of [3H]dihydroalprenolol with zinterol (a specific beta2-agonist) indicated that 72% of these sites were type beta2-receptors. beta-receptors were also present on granulosa cells. Stimulation of granulosa cells with luteinizing hormone or the beta-agonist isoproterenol increased the release of progesterone after 4 d in culture. These results suggest that the sympathetic nerves present in human ovary are coupled to beta-adrenergic receptors present in endocrine cells and, as in nonprimate mammals, appear to participate in the regulation of ovarian function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Agonists / pharmacology
  • Adult
  • Binding Sites
  • Calcium / pharmacology
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Dihydroalprenolol / metabolism
  • Dopamine / analysis
  • Electric Stimulation
  • Epinephrine / analysis
  • Ethanolamines / pharmacology
  • Female
  • Granulosa Cells / drug effects
  • Granulosa Cells / metabolism
  • Humans
  • Isoproterenol / pharmacology
  • Middle Aged
  • Norepinephrine / analysis
  • Norepinephrine / metabolism*
  • Ovary / chemistry
  • Ovary / metabolism*
  • Ovary / ultrastructure
  • Progesterone / metabolism
  • Receptors, Adrenergic, beta / drug effects
  • Receptors, Adrenergic, beta / metabolism
  • Tritium

Substances

  • Adrenergic beta-Agonists
  • Ethanolamines
  • Receptors, Adrenergic, beta
  • Tritium
  • Progesterone
  • Dihydroalprenolol
  • zinterol
  • Isoproterenol
  • Calcium
  • Dopamine
  • Norepinephrine
  • Epinephrine