Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

Br J Cancer. 2001 Nov 2;85(9):1372-82. doi: 10.1054/bjoc.2001.2074.


Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80-90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Blotting, Northern
  • Carcinoma, Renal Cell / genetics*
  • Cell Adhesion
  • Cell Survival
  • DNA Primers
  • DNA, Neoplasm / genetics
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Kidney Neoplasms / genetics*
  • Neovascularization, Pathologic
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Biomarkers, Tumor
  • DNA Primers
  • DNA, Neoplasm