Inhibition of Mycobacterium tuberculosis growth by saringosterol from Lessonia nigrescens

J Nat Prod. 2001 Nov;64(11):1463-4. doi: 10.1021/np010101q.

Abstract

Assay-guided fractionation of an antitubercular extract obtained from Lessonia nigrescens yielded the phytosterol saringosterol as its active component. No appreciable toxicity against Vero cells was observed for this compound. Saringosterol was also synthesized by oxidation of fucosterol. The MIC values for antitubercular activity of saringosterol and its 24S and 24R epimers were determined as 0.25, 1, and 0.125 microg/mL.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antitubercular Agents / chemical synthesis
  • Antitubercular Agents / chemistry
  • Antitubercular Agents / isolation & purification*
  • Antitubercular Agents / pharmacology
  • Cells, Cultured / drug effects
  • Chile
  • Chlorocebus aethiops
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Dose-Response Relationship, Drug
  • Molecular Structure
  • Mycobacterium tuberculosis / drug effects*
  • Nuclear Magnetic Resonance, Biomolecular
  • Oxidation-Reduction
  • Phaeophyceae / chemistry*
  • Stereoisomerism
  • Stigmasterol / analogs & derivatives*
  • Stigmasterol / chemical synthesis
  • Stigmasterol / chemistry
  • Stigmasterol / isolation & purification*
  • Stigmasterol / pharmacology
  • Vero Cells / drug effects

Substances

  • Antitubercular Agents
  • saringosterol
  • fucosterol
  • Stigmasterol