Genes required for ionizing radiation resistance in yeast

Nat Genet. 2001 Dec;29(4):426-34. doi: 10.1038/ng778.


The ability of Saccharomyces cerevisiae to tolerate ionizing radiation damage requires many DNA-repair and checkpoint genes, most having human orthologs. A genome-wide screen of diploid mutants homozygous with respect to deletions of 3,670 nonessential genes revealed 107 new loci that influence gamma-ray sensitivity. Many affect replication, recombination and checkpoint functions. Nearly 90% were sensitive to other agents, and most new genes could be assigned to the following functional groups: chromatin remodeling, chromosome segregation, nuclear pore formation, transcription, Golgi/vacuolar activities, ubiquitin-mediated protein degradation, cytokinesis, mitochondrial activity and cell wall maintenance. Over 50% share homology with human genes, including 17 implicated in cancer, indicating that a large set of newly identified human genes may have related roles in the toleration of radiation damage.

MeSH terms

  • Base Sequence
  • DNA Damage
  • DNA Primers
  • Gamma Rays
  • Genes, Fungal*
  • Mutation
  • Ploidies
  • Radiation Tolerance / genetics*
  • Recombination, Genetic
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / radiation effects*


  • DNA Primers