4-Hydroxybenzoate uptake in Klebsiella planticola strain DSZ1 is driven by DeltapH

Curr Microbiol. 2002 Jan;44(1):31-7. doi: 10.1007/s00284-001-0070-0.

Abstract

Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 x 10-6 (cpm/OD) h-1, and a rate of 4-HBA utilisation of 0.75 mmol h-1. Aerobically the 4HBA transport system is driven by gradient of protons (DeltapH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 microM with a Vmax value of 25 nmol/min.mg at pH 7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / analysis
  • Adenosine Triphosphate / metabolism
  • Anaerobiosis
  • Animals
  • Benzoates / metabolism*
  • Benzoates / pharmacology*
  • Biological Transport / drug effects*
  • Hydrogen-Ion Concentration*
  • Klebsiella / metabolism*
  • Membrane Potentials / drug effects
  • Parabens / metabolism*
  • Sodium Azide / metabolism

Substances

  • Benzoates
  • Parabens
  • Adenosine Triphosphate
  • Sodium Azide