Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses

J Biol Chem. 2002 Feb 15;277(7):5345-50. doi: 10.1074/jbc.M110052200. Epub 2001 Nov 29.

Abstract

Densin-180, a protein purified from the postsynaptic density fraction of the rat forebrain, is the founding member of a newly described family of proteins termed the LAP (leucine-rich repeats and PSD-95/Dlg-A/ZO-1 (PDZ) domains) family that plays essential roles in establishment of cell polarity. To identify Densin-180-binding proteins, we screened a yeast two-hybrid library using the carboxyl-terminal fragment of Densin-180 containing PDZ domain as bait, and we isolated delta-catenin/neural plakophilin-related armadillo repeat protein (NPRAP) as a Densin-180-interacting protein. delta-catenin/NPRAP, a member of the armadillo repeat family, is a nervous system-specific adherens junction protein originally discovered as an interactor with presenilin-1, a protein involved in Alzheimer's disease. Densin-180 PDZ domain binds the COOH terminus of delta-catenin/NPRAP containing the PDZ domain-binding sequence. Endogenous Densin-180 was co-immunoprecipitated with delta-catenin/NPRAP and N-cadherin. Although Densin-180 was reported to be a transmembrane protein, Densin-180 was not accessible to surface biotinylation in dissociated hippocampal neurons; hence Densin-180 may be a cytosolic protein. Densin-180 co-localized with delta-catenin/NPRAP at synapses in delta-catenin/NPRAP and may be involved in organization of the synaptic cell-cell junction through interaction with the delta-catenin/NPRAP-N-cadherin complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Armadillo Domain Proteins
  • Biotinylation
  • Brain / metabolism
  • Cadherins / metabolism
  • Catenins
  • Cell Adhesion Molecules
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Cloning, Molecular
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / metabolism*
  • Cytosol / metabolism
  • DNA, Complementary / metabolism
  • Delta Catenin
  • Gene Library
  • Hippocampus / cytology
  • Hippocampus / metabolism
  • Humans
  • Immunoblotting
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / metabolism
  • Neurons / metabolism
  • Phosphoproteins
  • Precipitin Tests
  • Protein Binding
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Proteins / metabolism
  • Sialoglycoproteins / chemistry*
  • Sialoglycoproteins / metabolism*
  • Synapses / metabolism
  • Transfection
  • Two-Hybrid System Techniques

Substances

  • Armadillo Domain Proteins
  • Cadherins
  • Catenins
  • Cell Adhesion Molecules
  • Ctnnd2 protein, rat
  • Cytoskeletal Proteins
  • DNA, Complementary
  • LRRC7 protein, human
  • Lrrc7 protein, rat
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Sialoglycoproteins
  • Delta Catenin

Associated data

  • GENBANK/AF434715