DNA probes using fluorescence resonance energy transfer (FRET): designs and applications

Biotechniques. 2001 Nov;31(5):1106-16, 1118, 1120-1. doi: 10.2144/01315rv02.


Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biosensing Techniques
  • DNA / metabolism
  • DNA Probes*
  • Energy Transfer
  • Humans
  • Mutation
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Spectrometry, Fluorescence / methods*


  • DNA Probes
  • DNA