Expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in serosal tissue of intraperitoneal organs and adhesions

Fertil Steril. 2001 Dec;76(6):1212-9. doi: 10.1016/s0015-0282(01)02875-8.

Abstract

Objective: To compare expression of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP (TIMP-1) in serosal tissue of intraperitoneal organs and adhesions.

Design: Prospective and cross-sectional study.

Setting: Academic research centers.

Patient(s): Patients undergoing abdominal or pelvic surgery.

Intervention(s): MMP-1 and TIMP-1 expression.

Main outcome measure(s): Expression of messenger ribonucleic acid (mRNA) and protein was measured by using quantitative reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay.

Result(s): Serosal tissue of intraperitoneal organs and adhesions express MMP-1 and TIMP-1 mRNA and protein at levels that are consistently varied with 10- to 10,000-fold and 2- to 10-fold higher TIMP, mRNA and protein, respectively. Parietal peritoneum, fallopian tubes and ovaries express higher MMP-1 mRNA levels compared with uterus and adhesions; the lowest expression is found in small and large bowels, subcutaneous tissue. and omentum. Expression of TIMP-1 mRNA was less variable; the highest level was found in the uterus and the lowest in subcutaneous tissue and small bowels. There was less variability in MMP-1 and TIMP-1 protein content than mRNA expression; ovaries and adhesions contained the highest MMP-1 and TIMP-1 levels, respectively, and peritoneum contained the lowest. The MMP-1 and TIMP-1 content and ratios further indicate limited MMP-1 proteolytic activity. Although tissues from premenopausal women express more MMP-1 and TIMP-1, expression did not differ by sex or age.

Conclusion(s): Because MMP-1 and TIMP-1 expression varies consistently among the serosal tissues of peritoneal organs and adhesions, and because tissue injury alters their expression, site-specific variations in expression of these substances may predispose a particular organ to develop more adhesions.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdomen / surgery
  • Adult
  • Aged
  • Aged, 80 and over
  • Cross-Sectional Studies
  • DNA, Complementary / chemistry
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Matrix Metalloproteinase 1 / biosynthesis*
  • Matrix Metalloproteinase 1 / chemistry
  • Matrix Metalloproteinase 1 / genetics
  • Middle Aged
  • Peritoneal Diseases / enzymology*
  • Peritoneal Diseases / pathology
  • Peritoneum / enzymology*
  • Peritoneum / pathology
  • Prospective Studies
  • RNA / chemistry
  • RNA / genetics
  • RNA / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Statistics, Nonparametric
  • Tissue Adhesions / enzymology*
  • Tissue Adhesions / pathology
  • Tissue Inhibitor of Metalloproteinase-1 / biosynthesis*
  • Tissue Inhibitor of Metalloproteinase-1 / chemistry
  • Tissue Inhibitor of Metalloproteinase-1 / genetics

Substances

  • DNA, Complementary
  • Tissue Inhibitor of Metalloproteinase-1
  • RNA
  • Matrix Metalloproteinase 1