Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CD1d

Nat Immunol. 2002 Jan;3(1):55-60. doi: 10.1038/ni740. Epub 2001 Dec 3.


For members of the CD1 family of beta(2)-microglobulin-associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD1 trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD1 that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD1d cytoplasmic tail deleted. Despite adequate surface CD1d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD1d cytoplasmic tail motif in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Motifs
  • Animals
  • Antigen Presentation / physiology*
  • Antigens / immunology
  • Antigens, CD1 / chemistry
  • Antigens, CD1 / genetics
  • Antigens, CD1 / immunology*
  • Antigens, CD1d
  • B-Lymphocytes / immunology
  • Biological Transport
  • Biotinylation
  • Clonal Deletion
  • Crosses, Genetic
  • Dendritic Cells / immunology
  • Endosomes / metabolism
  • Female
  • Galactosylceramides / immunology
  • Gene Targeting*
  • Hybridomas / immunology
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Phosphotyrosine / physiology*
  • Protein Transport
  • Receptors, Antigen, T-Cell, alpha-beta / immunology
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / immunology
  • Sequence Deletion
  • Specific Pathogen-Free Organisms
  • Stem Cells
  • T-Lymphocyte Subsets / immunology*
  • Thymus Gland / cytology*
  • Thymus Gland / immunology
  • Transfection


  • Antigens
  • Antigens, CD1
  • Antigens, CD1d
  • Galactosylceramides
  • Receptors, Antigen, T-Cell, alpha-beta
  • Recombinant Fusion Proteins
  • Phosphotyrosine