[Molecular studies in a population of children with bronchial asthma. I. Polymorphism in the promotor region of gene CD14]

Pneumonol Alergol Pol. 2001;69(5-6):265-72.
[Article in Polish]


Allergic asthma is associated with the recruitment of the inflammatory cells into the bronchial mucosa. Surface expression of CD14, a marker of activation and differentiation of macrophages/monocytes, was suggested to protect against Th-2 response.

Objective: To investigate clinical effects of genetic polymorphism of CD14 promoter in asthmatic children.

Methods: In ISAAC survey, 50 children with asthma were identified (wheezing in the last year, serum IgE level > 150 kIU/l, positive bronchial challenge test with aerolized hypertonic saline) and 73 children without the above signs. Age range of surveyed children was 13-14 years. Genotypic pattern of CD14 promoter -159 C to T transition was assessed by RFLP method.

Results: There was no difference in the allelic (0.36 vs. 0.38 for -159T) or genotype frequencies (0.12 vs. 0.15 for -159TT) of CD14 polymorphism between allergic asthmatics and controls. Moreover, there was no relationship between CD14 genotype and serum IgE level or bronchial hyper-responsiveness.

Conclusion: Our results do not confirm the association of CD14 polymorphism (promoter -159 C to T transition) with asthma in Polish children.

MeSH terms

  • Adolescent
  • Asthma / epidemiology
  • Asthma / genetics*
  • Asthma / immunology
  • Bronchi / immunology
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease / genetics*
  • Genetics, Population
  • Genotype
  • Humans
  • Lipopolysaccharide Receptors / genetics*
  • Lipopolysaccharide Receptors / immunology
  • Male
  • Neutrophil Infiltration
  • Poland / epidemiology
  • Polymorphism, Restriction Fragment Length*


  • Lipopolysaccharide Receptors