Identification and characterization of AGTRAP, a human homolog of murine Angiotensin II Receptor-Associated Protein (Agtrap)

Int J Biochem Cell Biol. 2002 Jan;34(1):93-102. doi: 10.1016/s1357-2725(01)00094-2.


Several classes of cytoplasmic proteins have been found to interact specifically with the carboxyl-terminal cytoplasmic region of the angiotensin II type 1 (AT(1)) receptor to regulate different aspects of AT(1) receptor physiology. The murine Angiotensin II Receptor-Associated Protein (Agtrap) is a new member of them. We have recently cloned a new human gene cDNA that codes for a homolog of the murine Agtrap protein from a human fetal brain cDNA library. The deduced polypeptide product of the cDNA is 22 kDa in size, and its DNA and amino acid sequences are 85 and 77% identical to those of the mouse Agtrap gene, respectively. Hence we have named it the human Angiotensin II Receptor-Associated Protein (AGTRAP) gene. The mRNA of AGTRAP was most abundantly expressed in kidney, heart, pancreas and thyroid. Using the yeast two-hybrid screening of a human fetal brain cDNA library, we have identified a new interaction partner of the human AGTRAP protein, RACK1 (Receptor of Activated Protein C Kinase). The AGTRAP-RACK1 interaction was confirmed by GST fusion protein pull-down assays, co-immunoprecipitation and surface plasmon resonance. We suggest that the AGTRAP-RACK1 interaction may help to recruit signaling complex to the AT(1) receptor to affect AT(1) receptor signaling.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Humans
  • In Vitro Techniques
  • Mice
  • Molecular Sequence Data
  • Peptides / metabolism
  • Pregnancy
  • Protein Binding
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptor, Angiotensin, Type 1
  • Receptors for Activated C Kinase
  • Receptors, Angiotensin / chemistry
  • Receptors, Angiotensin / genetics*
  • Receptors, Angiotensin / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Species Specificity
  • Tissue Distribution
  • Two-Hybrid System Techniques


  • AGTRAP protein, human
  • Adaptor Proteins, Signal Transducing
  • DNA, Complementary
  • Peptides
  • RNA, Messenger
  • Receptor, Angiotensin, Type 1
  • Receptors for Activated C Kinase
  • Receptors, Angiotensin
  • Recombinant Proteins
  • peptide I

Associated data

  • GENBANK/AF312374