A novel spermatogenesis-related factor-1 gene expressed in maturing rat testis

Biochem Biophys Res Commun. 2001 Dec 14;289(4):888-93. doi: 10.1006/bbrc.2001.6048.

Abstract

A rat gene with testis-specific expression coinciding with spermatogenesis was cloned by differential display. This spermatogenesis-related factor-1 (SRF-1) gene was not expressed in other organs. Testicular expression was detected from 5 weeks of age and increased up to 15 weeks; this level of expression was maintained for 63 weeks. The 750-bp cloned gene was coded for an open reading frame of 202 amino acids. According to in situ hybridization at 7 weeks, this gene was expressed mainly in spermatocyte. The gene product may function as a molecular motor in meiosis, as the deduced amino acid sequence showed partial homology with kinesin-related proteins. The action of this gene and its product with respect to division of reproductive cells requires further investigation.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Binding Proteins / genetics
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization
  • Kinesin
  • Male
  • Molecular Motor Proteins / genetics
  • Molecular Sequence Data
  • Muscle Proteins / genetics
  • Open Reading Frames
  • Proteins / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Spermatogenesis / genetics*
  • Testis / growth & development*
  • Testis / metabolism*

Substances

  • Calcium-Binding Proteins
  • DNA, Complementary
  • Molecular Motor Proteins
  • Muscle Proteins
  • Proteins
  • Spata6 protein, rat
  • kinesin-II
  • Kinesin

Associated data

  • GENBANK/AB046606