Sequence of the 50-kb Conjugative Multiresistance Plasmid pRE25 From Enterococcus Faecalis RE25

Plasmid. 2001 Nov;46(3):170-87. doi: 10.1006/plas.2001.1544.

Abstract

The complete 50,237-bp DNA sequence of the conjugative and mobilizing multiresistance plasmid pRE25 from Enterococcus faecalis RE25 was determined. The plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. Chloramphenicol acetyltransferase and the 23S RNA methylase are identical to gene products of the broad-host-range plasmid pIP501 from Streptococcus agalactiae. In addition, a 30.5-kb segment is almost identical to pIP501. Genes encoding an aminoglycoside 6-adenylyltransferase, a streptothricin acetyltransferase, and an aminoglycoside phosphotransferase are arranged in tandem on a 7.4-kb fragment as previously reported in Tn5405 from Staphylococcus aureus and in pJH1 from E. faecalis. One interrupted and five complete IS elements as well as three replication genes were also identified. pRE25 was transferred by conjugation to E. faecalis, Listeria innocua, and Lactococcus lactis by means of a transfer region that appears similar to that of pIP501. It is concluded that pRE25 may contribute to the further spread of antibiotic-resistant microorganisms via food into the human community.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Bacterial Agents / classification
  • Anti-Bacterial Agents / pharmacology
  • Clostridium / genetics
  • Conjugation, Genetic
  • DNA Replication
  • DNA Transposable Elements / genetics
  • DNA, Bacterial / genetics
  • Drug Resistance / genetics*
  • Drug Resistance, Multiple / genetics*
  • Enterococcus faecalis / genetics*
  • Evolution, Molecular
  • Food Contamination
  • Food Microbiology
  • Genes, Bacterial
  • Lactococcus / genetics
  • Meat / microbiology
  • Molecular Sequence Data
  • Open Reading Frames
  • R Factors / genetics*
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Streptococcus agalactiae / genetics

Substances

  • Anti-Bacterial Agents
  • DNA Transposable Elements
  • DNA, Bacterial

Associated data

  • GENBANK/X92945