Molecular basis of Tn-polyagglutinability

Vox Sang. 1975;29(1):36-50. doi: 10.1111/j.1423-0410.1975.tb00475.x.

Abstract

Spectrophotometric and gas-liquid chromatographic analyses on the carbohydrate moiety of tryptic erythrocyte glycopeptides from persons with Tn-syndrome reveal a selective lowering of the galactose and sialic acid content, the degree being dependent on the percentage of polyagglutinable cells. Alkaline borohydride specifically releases N-acetylgalactosaminitol, and the amount is correlated to the percentage of pathological acetylgalactosaminitol, and the amount is correlated to the percentage of pathological erythrocytes. It is concluded that the alkali-labile carbohydrate chains of Tn-polyagglutinable red cells solely consist of N-acetylgalactosamine linked to serine or threonine. Experiments with heterophile agglutinins whose specificity is known are in line with the above-mentioned results. As judged from SDS-polyacrylamide gel electrophoresis the three major membrane glycoproteins are affected to a different extent by the defect.

MeSH terms

  • Anemia, Hemolytic / immunology*
  • Antibodies, Heterophile / analysis
  • Blood Glucose / analysis
  • Blood Protein Electrophoresis
  • Cell Membrane / immunology
  • Chromatography, Gas
  • Electrophoresis, Polyacrylamide Gel
  • Erythrocytes / analysis
  • Glycopeptides / analysis
  • Hemagglutination Tests
  • Hemagglutination*
  • Hexoses / analysis
  • Humans
  • Leukopenia / immunology*
  • Molecular Weight
  • Sialic Acids / analysis
  • Sodium Dodecyl Sulfate
  • Syndrome
  • Thrombocytopenia / immunology*
  • Trypsin

Substances

  • Antibodies, Heterophile
  • Blood Glucose
  • Glycopeptides
  • Hexoses
  • Sialic Acids
  • Sodium Dodecyl Sulfate
  • Trypsin