Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells

Dev Cell. 2001 Dec;1(6):759-70. doi: 10.1016/s1534-5807(01)00088-0.


Proteolytic cleavage of the cohesin subunit Scc1 is a consistent feature of anaphase onset, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres. We describe the effects of genetic deletion of Scc1 in chicken DT40 cells. Scc1 loss caused premature sister chromatid separation but did not disrupt chromosome condensation. Scc1 mutants showed defective repair of spontaneous and induced DNA damage. Scc1-deficient cells frequently failed to complete metaphase chromosome alignment and showed chromosome segregation defects, suggesting aberrant kinetochore function. Notably, the chromosome passenger INCENP did not localize normally to centromeres, while the constitutive kinetochore proteins CENP-C and CENP-H behaved normally. These results suggest a role for Scc1 in mitotic regulation, along with cohesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Cell Cycle / physiology*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism
  • Chickens
  • Chromatids / metabolism*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism
  • DNA Repair
  • Doxycycline / pharmacology
  • Flow Cytometry
  • Fungal Proteins
  • Humans
  • In Situ Hybridization, Fluorescence
  • Kinetochores / metabolism*
  • Macromolecular Substances
  • Microscopy, Atomic Force
  • Microscopy, Fluorescence
  • Nuclear Proteins / metabolism
  • Phenotype
  • Phosphoproteins
  • Protein Subunits
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae Proteins


  • Anti-Bacterial Agents
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • INCENP protein, human
  • MCD1 protein, S cerevisiae
  • Macromolecular Substances
  • Nuclear Proteins
  • Phosphoproteins
  • Protein Subunits
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • cohesins
  • Doxycycline