Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes

Methods Cell Sci. 2001;23(1-3):171-4. doi: 10.1007/978-94-010-0330-8_18.

Abstract

HP1 proteins are conserved non histone chromosomal proteins involved in the epigenetic repression of transcription. Three HP1 proteins, HP1alpha, HP1beta and HP1gamma are expressed in mammalian cells. Polyclonal antibodies directed against peptides specific for HP1alpha and HP1gamma were elicited in rabbits, affinity purified, then used to localize both proteins on spreads of unfixed metaphasic chromosomes. We show here, by conventional and confocal microscopy, that both proteins are localized at centromeres and pericentromeres, and that HP1gamma is also present on euchromatic sites of chromosome arms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Centromere / ultrastructure*
  • Chromosomal Proteins, Non-Histone / isolation & purification*
  • Chromosomes, Human / ultrastructure*
  • HeLa Cells
  • Humans
  • Metaphase

Substances

  • Chromosomal Proteins, Non-Histone
  • heterochromatin-specific nonhistone chromosomal protein HP-1