Intravenous delivery of naked plasmid DNA for in vivo cytokine expression

Biochem Biophys Res Commun. 2001 Dec 21;289(5):1088-92. doi: 10.1006/bbrc.2001.6100.


We previously demonstrated that electroporation-mediated cytokine gene delivery into muscle is an effective approach for long-term systemic delivery of cytokines. Here we show that hydrodynamics-based gene delivery into mice by intravenous administration of naked plasmid DNA is a more efficient procedure for expressing cytokines in vivo. A large volume of Ringer's solution containing an interleukin-10 (IL-10) expression plasmid pCAGGS-IL10 was rapidly injected into the tail vein of mice. Serum IL-10 levels increased in a dose-dependent manner with a saturation level (50.8 +/- 12.1 microg/ml) 10,000-fold higher than we obtained by the electroporation-mediated method. High levels of serum IL-10 were sustained for at least 2 weeks following a single injection. These results demonstrate that hydrodynamics-based gene delivery could induce sustained high-level expression of cytokines, which would be useful for further studies of cytokine function in vivo and the development of novel immunotherapeutic strategies for systemic cytokine gene therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cytokines / genetics*
  • Gene Expression
  • Gene Transfer Techniques*
  • Genetic Therapy
  • Injections, Intravenous
  • Interleukin-10 / blood
  • Interleukin-10 / genetics*
  • Lac Operon
  • Male
  • Mice
  • Mice, Inbred ICR
  • Mice, Transgenic
  • Plasmids / administration & dosage*
  • Plasmids / genetics*
  • Transfection


  • Cytokines
  • Interleukin-10