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. 2002 Jan;184(1):318-22.
doi: 10.1128/jb.184.1.318-322.2002.

Sequencing of Flagellin Genes From Natrialba Magadii Provides New Insight Into Evolutionary Aspects of Archaeal Flagellins

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Free PMC article

Sequencing of Flagellin Genes From Natrialba Magadii Provides New Insight Into Evolutionary Aspects of Archaeal Flagellins

Inna Serganova et al. J Bacteriol. .
Free PMC article

Abstract

We have determined the nucleotide sequence of a flagellin gene locus from the haloalkaliphilic archaeon Natrialba magadii, identified the gene products among proteins forming flagella, and demonstrated cotranscription of the genes. Based on the sequence analysis we suggest that different regions of the genes might have distinct evolutionary histories including possible genetic exchange with bacterial flagellin genes.

Figures

FIG. 1.
FIG. 1.
Organization of N. magadii flagellin operon. Restriction sites used for cloning and inverse PCR are indicated. Bent arrow, position of the transcription start point.
FIG. 2.
FIG. 2.
Homology between N. magadii and archaeal flagellins. (A) Multiple sequence alignment between N. magadii and representative archaeal flagellins was performed using ClustalW (39). Identical amino acids along with conserved substitutions were shaded with GeneDoc software (K. B. Nicholas and H. B. Nicholas, Jr., unpublished data). Amino acids similar in all proteins are white on a black background, and similar residues represented in >50% of the sequences are shaded in gray. The origins of the sequences were as follows: B1Nm to B4Nm, N. magadii; B1Hh, H. halobium (15); B2Mj, M. jannaschii (6); B2Ph, Pyrococcus horikoshii (22); B1Arf, Archaeoglobus fulgidus (24); B1Aerp, Aeropyrum pernix (23). (B) Comparison of the central parts of N. magadii FlaB3 and E. coli DEC 2a flagellins performed using BLAST (2).
FIG. 3.
FIG. 3.
Transcription of the flagellin gene cluster in N. magadii. (A) Northern blot analysis of flagellin mRNA. The positions of RNA markers (Promega Corporation, Madison, Wis.) are indicated on the left. (B) Mapping of the 5′ terminus of the flaB transcript by primer extension. The primer extension product (arrow [lane P]) was separated by electrophoresis in a denaturing 6% polyacrylamide gel along with the sequence ladders generated with the same oligonucleotide (lanes A, G, C, and T). (C) Nucleotide sequence of the flaB promoter region. Bent arrow, transcription start point. The putative transcription signals are boxed, and halobacterial consensus sequences are shown under them. The putative ribosome binding site is underlined.

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