Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells

Acta Pharmacol Sin. 2001 Apr;22(4):335-9.

Abstract

Aim: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304).

Methods: VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization.

Results: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time- and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells.

Conclusion: LPC could induce a strong expression of VEGF in ECV304 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / metabolism
  • Gene Expression / drug effects*
  • Humans
  • Lysophosphatidylcholines / pharmacology*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects
  • Umbilical Veins / cytology
  • Vascular Endothelial Growth Factor A / biosynthesis*
  • Vascular Endothelial Growth Factor A / genetics

Substances

  • Lysophosphatidylcholines
  • RNA, Messenger
  • Vascular Endothelial Growth Factor A