AMPA-Induced suppression of oligodendroglial gene expression in neonatal rat brain

Brain Res Dev Brain Res. 2001 Dec 31;132(2):175-8. doi: 10.1016/s0165-3806(01)00306-6.


Oligodendroglia are susceptible to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated excitotoxicity. We hypothesized that analysis of oligodendroglial gene expression would provide a sensitive indicator of acute excitotoxic oligodendroglial injury in the immature brain. AMPA (2 nmol) was injected intracerebroventricularly in 7-day-old rats. Proteolipid protein and myelin basic protein encoding mRNAs were evaluated 24-48 h later by semi-quantitative reverse transcription-polymerase chain reaction assays. AMPA markedly suppressed brain tissue content of oligodendroglial mRNAs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Excitatory Amino Acid Agonists / pharmacology*
  • Gene Expression / drug effects
  • Injections, Intraventricular
  • Myelin Basic Protein / genetics*
  • Myelin Proteolipid Protein / genetics*
  • Oligodendroglia / physiology*
  • RNA, Messenger / analysis
  • Rats
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology*


  • Excitatory Amino Acid Agonists
  • Myelin Basic Protein
  • Myelin Proteolipid Protein
  • RNA, Messenger
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid