The mouse enhancer element D6 directs Cre recombinase activity in the neocortex and the hippocampus

Mech Dev. 2002 Jan;110(1-2):179-82. doi: 10.1016/s0925-4773(01)00597-4.


We have created a transgenic mouse line that expresses Cre recombinase under the control of the novel mouse promoter/enhancer D6. We describe the expression pattern of D6-Cre in a Gtrosa26 reporter background as assayed by LacZ activity. The enhancer activity starts at 10.5 days post-coitum in the telencephalon and is at the later embryonic stages highly restricted to the hippocampus and the neocortex. In adult mice D6-derived cells are found in cortical layers II-VI, in the granular cells of the dentate gyrus and in hippocampal fields CA1-CA3. D6-Cre activity is also detected in the ependymal and subependymal zone of the lateral ventricles which is known to harbor neural stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Genes, Reporter
  • Hippocampus / embryology*
  • Hippocampus / enzymology*
  • Hippocampus / growth & development
  • Immunohistochemistry
  • Integrases / genetics*
  • Lac Operon
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Neocortex / embryology*
  • Neocortex / enzymology*
  • Neocortex / growth & development
  • Neurons / enzymology
  • Stem Cells / enzymology
  • Viral Proteins / genetics*


  • Viral Proteins
  • Cre recombinase
  • Integrases