PUMA-G, an IFN-gamma-inducible gene in macrophages is a novel member of the seven transmembrane spanning receptor superfamily

Eur J Immunol. 2001 Dec;31(12):3714-25. doi: 10.1002/1521-4141(200112)31:12<3714::aid-immu3714>3.0.co;2-1.


IFN-gamma is a key immunoregulatory cytokine that plays a predominant role in innate immunity. By employing PCR-Select to search for genes differentially expressed in IFN-gamma/TNF-alpha stimulated macrophages, we identified a novel IFN-gamma-induced transcript designated PUMA-G (protein up-regulated in macrophages by IFN-gamma). PUMA-G codes for a protein with seven transmembrane helices, a feature commonly shared with the G protein-coupled receptor superfamily (GPCR). The PUMA-G protein is most similar to the human orphan GPCR HM74 (73 % identity) and GPR31 (30 % identity). PUMA-G mRNA was readily induced in macrophages after stimulation with IFN-gamma, LPS, polyIC and CpG oligonucleotides. In vivo PUMA-G was up-regulated in mice suffering from microbial sepsis or from Listeria monocytogenes infection. Characterization of the genomic locus revealed an intronless PUMA-G open reading frame. Genomic Southern blot analysis indicates that PUMA-G is a single-copy gene. PUMA-G maps to mouse chromosome 5F. Confocal microscopy of transiently transfected 264.7 RAW macrophages and 293T cells with a PUMA-G-EGFP fusion construct showed predominant fluorescence at the cell surface, suggesting a localization at the cell membrane. Taken together, our data indicate that PUMA-G is a new inducible representative of GPCR, with potential importance in macrophage functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Interferon-gamma / pharmacology*
  • Listeriosis / metabolism
  • Liver / metabolism
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Receptors, Cell Surface / analysis*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology
  • Spleen / metabolism


  • RNA, Messenger
  • Receptors, Cell Surface
  • Interferon-gamma

Associated data

  • GENBANK/AJ300198