An extensive monoclonal antibody panel for the phenotyping of leukocyte subsets in the common marmoset and the cotton-top tamarin

Cytometry. 2001 Dec 1;45(4):294-303. doi: 10.1002/1097-0320(20011201)45:4<294::aid-cyto10002>3.0.co;2-c.

Abstract

New World monkeys are valuable animal models to study human diseases. To determine the phenotype of cells involved in immune responses, we used flow cytometry to screen a large panel of anti-human monoclonal antibodies (mAb) for cross-reactivity with cells of the common marmoset and the cotton-top tamarin. Certain antigens (e.g., CD2, CD8, CD20) are well conserved. However, CD10, CD23, and CD33 showed a clear discrepancy in their reaction patterns in both species, indicating that significant differences on the epitope level occurred during evolution. Epstein-Barr virus-transformed B-cell lines were shown to be a valuable tool for screening B-cell-specific reagents. In some cases, fluorescein isothiocyanate (FITC) and phycoerythrin (PE) modification of mAbs had a negative effect on the binding capacity, which stressed the importance of choosing the right label. Despite the fact that some CD antigens were not detected, adequate numbers of cross-reactive mAbs were identified to perform extensive studies on immunological functions in both the common marmoset and the cotton-top tamarin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis*
  • Antibody Specificity
  • Antigens, CD / immunology
  • Callithrix / blood*
  • Cross Reactions
  • Female
  • Flow Cytometry
  • Immunophenotyping
  • Lymphocyte Subsets / classification
  • Lymphocyte Subsets / cytology
  • Lymphocyte Subsets / immunology*
  • Male
  • Saguinus / blood*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD