Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates

Cell. 2001 Dec 14;107(6):739-50. doi: 10.1016/s0092-8674(01)00596-7.

Abstract

In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs. Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription factor to the daughter nucleus. Ectopic localization of active Ace2 to mother nuclei is sufficient to activate daughter-specific genes in mothers. Eight genes are daughter-specific under the tested conditions, while two are daughter-specific only in saturated cultures. Some daughter-specific gene products contribute to cell separation by degrading the cell wall. These experiments define programs of gene expression specific to daughters and describe how those programs are controlled.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Active Transport, Cell Nucleus / physiology
  • Animals
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Division / physiology*
  • Chitinases / genetics
  • Chitinases / metabolism
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal / genetics
  • Intracellular Signaling Peptides and Proteins
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Phylogeny
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • ACE2 protein, S cerevisiae
  • ASH1 protein, S cerevisiae
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • EGT2 protein, S cerevisiae
  • Fungal Proteins
  • Intracellular Signaling Peptides and Proteins
  • MOB2 protein, S cerevisiae
  • Phosphoproteins
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • CBK1 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases
  • CTS1 protein, S cerevisiae
  • Chitinases