Transcription pattern of ribosomal protein L26 during anoxia exposure in Littorina littorea

J Exp Zool. 2001 Dec 1;290(7):759-68. doi: 10.1002/jez.1126.

Abstract

Differential screening of a hepatopancreas cDNA library derived from the marine snail Littorina littorea yielded a 421-bp clone coding for ribosomal protein L26 that was up-regulated during anoxia exposure. The deduced amino acid sequence, containing 144 residues with a predicted molecular weight of 17 kDa, showed 80% amino acid sequence identity to the mammalian ribosomal protein L26. Analysis of hepatopancreas and foot muscle samples from a time course of anoxia exposure showed a maximal transcript increase of 4- and 3-fold after 96 hr and 48 hr, respectively, relative to normoxic animals, with a subsequent decrease in transcript levels during normoxic recovery. Nuclear run-off assays confirmed the observed transcriptional up-regulation of L26 during anoxia. Organ culture experiments were performed to determine a possible pathway of up-regulation of L26, with data indicating a putative role for cGMP in signal transduction. The transcriptional up-regulation of L26 during anoxia may stabilize the existing mRNA pool, via a possible cGMP-mediated signaling cascade, until oxygen reappears and protein synthesis resumes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Animals
  • Cyclic GMP / pharmacology
  • Digestive System
  • Hypoxia*
  • Muscles / physiology
  • RNA, Messenger
  • Ribosomal Proteins / biosynthesis*
  • Signal Transduction
  • Snails / physiology*
  • Transcription, Genetic*
  • Up-Regulation

Substances

  • RNA, Messenger
  • Ribosomal Proteins
  • Cyclic GMP