Abstract
14-3-3 proteins comprise a family of highly conserved proteins that bind more than 60 different, mostly phosphorylated, proteins. The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2, encoding 14-3-3 proteins. Disruption of both genes together is lethal. In this study we constructed a mutant with a single, temperature-sensitive bmh allele. Recessive mutations in SIN4 and RTG3 can suppress the temperature-sensitive phenotype of this mutant. These genes encode a global transcriptional regulator and a basic helix-loop-helix transcription factor, respectively. The yeast 14-3-3 proteins were shown to bind to the Rtg3 protein. Overexpression of RTG3 is lethal even in wild-type cells. These genetic and biochemical data are consistent with a model in which the 14-3-3 proteins are required to keep the Rtg3 protein in an inactive state, which is (one of) the essential function(s) of the 14-3-3 proteins.
Copyright 2001 John Wiley & Sons, Ltd.
MeSH terms
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14-3-3 Proteins
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Antifungal Agents / pharmacology
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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DNA-Binding Proteins / physiology*
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Enzyme-Linked Immunosorbent Assay
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Fungal Proteins / genetics*
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Fungal Proteins / physiology*
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Gene Expression Regulation, Fungal / genetics*
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Gene Expression Regulation, Fungal / physiology
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Mutagenesis
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Polymerase Chain Reaction
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Recombinant Proteins
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins*
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Sirolimus / pharmacology
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Transcription Factors*
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Tyrosine 3-Monooxygenase / physiology*
Substances
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14-3-3 Proteins
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Antifungal Agents
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BMH1 protein, S cerevisiae
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BMH2 protein, S cerevisiae
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Fungal Proteins
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RTG3 protein, S cerevisiae
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Tyrosine 3-Monooxygenase
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Sirolimus