14-3-3 Proteins are essential for regulation of RTG3-dependent transcription in Saccharomyces cerevisiae

Yeast. 2001 Dec;18(16):1479-91. doi: 10.1002/yea.765.


14-3-3 proteins comprise a family of highly conserved proteins that bind more than 60 different, mostly phosphorylated, proteins. The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2, encoding 14-3-3 proteins. Disruption of both genes together is lethal. In this study we constructed a mutant with a single, temperature-sensitive bmh allele. Recessive mutations in SIN4 and RTG3 can suppress the temperature-sensitive phenotype of this mutant. These genes encode a global transcriptional regulator and a basic helix-loop-helix transcription factor, respectively. The yeast 14-3-3 proteins were shown to bind to the Rtg3 protein. Overexpression of RTG3 is lethal even in wild-type cells. These genetic and biochemical data are consistent with a model in which the 14-3-3 proteins are required to keep the Rtg3 protein in an inactive state, which is (one of) the essential function(s) of the 14-3-3 proteins.

MeSH terms

  • 14-3-3 Proteins
  • Antifungal Agents / pharmacology
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins / physiology*
  • Enzyme-Linked Immunosorbent Assay
  • Fungal Proteins / genetics*
  • Fungal Proteins / physiology*
  • Gene Expression Regulation, Fungal / genetics*
  • Gene Expression Regulation, Fungal / physiology
  • Mutagenesis
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Sirolimus / pharmacology
  • Transcription Factors*
  • Tyrosine 3-Monooxygenase / physiology*


  • 14-3-3 Proteins
  • Antifungal Agents
  • BMH1 protein, S cerevisiae
  • BMH2 protein, S cerevisiae
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Fungal Proteins
  • RTG3 protein, S cerevisiae
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Tyrosine 3-Monooxygenase
  • Sirolimus