Expression pattern in brain of TASK-1, TASK-3, and a tandem pore domain K(+) channel subunit, TASK-5, associated with the central auditory nervous system

Mol Cell Neurosci. 2001 Dec;18(6):632-48. doi: 10.1006/mcne.2001.1045.


TWIK-related acid-sensitive K(+) (TASK) channels contribute to setting the resting potential of mammalian neurons and have recently been defined as molecular targets for extracellular protons and volatile anesthetics. We have isolated a novel member of this subfamily, hTASK-5, from a human genomic library and mapped it to chromosomal region 20q12-20q13. hTASK-5 did not functionally express in Xenopus oocytes, whereas chimeric TASK-5/TASK-3 constructs containing the region between M1 and M3 of TASK-3 produced K(+) selective currents. To better correlate TASK subunits with native K(+) currents in neurons the precise cellular distribution of all TASK family members was elucidated in rat brain. A comprehensive in situ hybridization analysis revealed that both TASK-1 and TASK-3 transcripts are most strongly expressed in many neurons likely to be cholinergic, serotonergic, or noradrenergic. In contrast, TASK-5 expression is found in olfactory bulb mitral cells and Purkinje cells, but predominantly associated with the central auditory pathway. Thus, TASK-5 K(+) channels, possibly in conjunction with auxiliary proteins, may play a role in the transmission of temporal information in the auditory system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Auditory Pathways / cytology
  • Auditory Pathways / metabolism*
  • Base Sequence / genetics
  • Brain / cytology
  • Brain / metabolism*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Gene Expression Regulation / physiology*
  • Humans
  • In Situ Hybridization
  • Membrane Potentials / genetics
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Neurons / cytology
  • Neurons / metabolism*
  • Oocytes / cytology
  • Oocytes / metabolism
  • Potassium Channels / genetics*
  • Potassium Channels / metabolism
  • Potassium Channels, Tandem Pore Domain*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Xenopus


  • DNA, Complementary
  • KCNK15 protein, human
  • KCNK9 protein, human
  • Kcnk15 protein, rat
  • Kcnk9 protein, rat
  • Nerve Tissue Proteins
  • Potassium Channels
  • Potassium Channels, Tandem Pore Domain
  • RNA, Messenger
  • potassium channel subfamily K member 3