Regulation of growth-regulated oncogene alpha expression by estrogen in human endothelial cells

Acta Pharmacol Sin. 2001 Nov;22(11):1003-6.

Abstract

Aim: To study the effect of estrogen on expression of growth-regulated oncogene alpha (GROalpha) in human umbilical vein endothelial cells (HUVEC) in vitro.

Methods: Expressions of CXC chemokine GROalpha mRNA and protein were measured by Northern blotting assay and ELISA, respectively. The physiological significance of GROalpha expression was tested by static cell adhesion assay.

Results: Both the GROalpha mRNA and protein levels decreased markedly after HUVEC were exposured to 17beta-estradiol (2 0.05 micromol/L. Moreover, the inhibition of the protein was depended on the concentration of 17beta-estradiol. Tamoxifen (0.1 micromol/L), an estrogen receptor alpha antagonist, alone did not affect GROalpha protein expression, but can reverse the E2 -induced inhibition of GROalpha protein expression (by up to 50 %) and the binding of U937 cells to E2-treated HUVEC (by up to 40 %).

Conclusion: Estrogen might functionally down-regulates GROalpha expression through estrogen receptor alpha on endothelial cells.

MeSH terms

  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokines / biosynthesis*
  • Chemokines / genetics
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / genetics
  • Down-Regulation
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / metabolism
  • Estradiol / pharmacology*
  • Gene Expression Regulation / drug effects*
  • Humans
  • Intercellular Signaling Peptides and Proteins / biosynthesis*
  • Intercellular Signaling Peptides and Proteins / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Receptors, Estrogen / antagonists & inhibitors
  • Tamoxifen / pharmacology
  • Umbilical Veins / cytology

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Intercellular Signaling Peptides and Proteins
  • RNA, Messenger
  • Receptors, Estrogen
  • Tamoxifen
  • Estradiol