Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins

Brain Res Mol Brain Res. 2001 Dec 30;97(2):186-9. doi: 10.1016/s0169-328x(01)00303-5.

Abstract

The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.

MeSH terms

  • Acetylcysteine / analogs & derivatives*
  • Acetylcysteine / pharmacology
  • Animals
  • Carrier Proteins / analysis
  • Carrier Proteins / metabolism*
  • Cells, Cultured
  • Cysteine Endopeptidases / metabolism
  • Cysteine Proteinase Inhibitors / pharmacology
  • Hippocampus / cytology
  • Homer Scaffolding Proteins
  • Immunohistochemistry
  • Leupeptins / pharmacology
  • Multienzyme Complexes / antagonists & inhibitors*
  • Multienzyme Complexes / metabolism
  • Neuronal Plasticity / physiology
  • Neurons / chemistry
  • Neurons / metabolism*
  • Neuropeptides / analysis
  • Neuropeptides / metabolism*
  • Proteasome Endopeptidase Complex
  • Synapses / chemistry
  • Synapses / metabolism*
  • Synaptophysin / analysis

Substances

  • Carrier Proteins
  • Cysteine Proteinase Inhibitors
  • Homer Scaffolding Proteins
  • Leupeptins
  • Multienzyme Complexes
  • Neuropeptides
  • Synaptophysin
  • lactacystin
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde
  • Acetylcysteine