Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced

FEMS Microbiol Lett. 2001 Dec 18;205(2):323-8. doi: 10.1016/s0378-1097(01)00490-6.

Abstract

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Fructans / biosynthesis
  • Fructans / chemistry
  • Fructans / metabolism*
  • Hexosyltransferases / chemistry
  • Hexosyltransferases / isolation & purification
  • Hexosyltransferases / metabolism*
  • Lactobacillus / enzymology*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Molecular Weight
  • Polysaccharides, Bacterial / biosynthesis
  • Polysaccharides, Bacterial / metabolism*
  • Sequence Alignment
  • Sucrose / metabolism

Substances

  • Fructans
  • Polysaccharides, Bacterial
  • Sucrose
  • levan
  • Hexosyltransferases
  • levan fructotransferase
  • levansucrase