Estimating absolute rates of molecular evolution and divergence times: a penalized likelihood approach

Mol Biol Evol. 2002 Jan;19(1):101-9. doi: 10.1093/oxfordjournals.molbev.a003974.


Rates of molecular evolution vary widely between lineages, but quantification of how rates change has proven difficult. Recently proposed estimation procedures have mainly adopted highly parametric approaches that model rate evolution explicitly. In this study, a semiparametric smoothing method is developed using penalized likelihood. A saturated model in which every lineage has a separate rate is combined with a roughness penalty that discourages rates from varying too much across a phylogeny. A data-driven cross-validation criterion is then used to determine an optimal level of smoothing. This criterion is based on an estimate of the average prediction error associated with pruning lineages from the tree. The methods are applied to three data sets of six genes across a sample of land plants. Optimally smoothed estimates of absolute rates entailed 2- to 10-fold variation across lineages.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Evolution, Molecular*
  • Genes, Plant / genetics*
  • Genetic Variation
  • Likelihood Functions
  • Models, Genetic
  • Models, Statistical*
  • Phylogeny
  • Plants / genetics*
  • Reproducibility of Results
  • Time Factors