The immediate effects of intense aerobic exercise on the composition and oxidizability of low- (LDL) and high-density lipoproteins (HDL) were studied in 11 male athletes. Plasma parameters known to affect lipoprotein oxidizability were also evaluated. Lipophilic antioxidants, including alpha-tocopherol and carotenoids, paraoxonase and malondialdehyde (MDA) in plasma remained unchanged after exercise. Increases in the concentration of uric acid, bilirubin and ascorbic acid after the race resulted in a significant increase in total antioxidant serum capacity. LDL, but not HDL, increased its "in vitro"-induced susceptibility to oxidation and the proportion of electronegative LDL (LDL-). The ability of HDL to inhibit the oxidation of LDL remained unchanged after exercise. The enhanced oxidizability of LDL was not explained by increments in its aldehyde content or by decrements in antioxidants. The major compositional change in LDL was an increase in non-esterified fatty acid (NEFA) content (from 4.00+/-1.24 to 19.00+/-14.18 mol NEFA/mol apoB). NEFA also increased in plasma and HDL. "In vitro" experiments showed that incubation of LDL with increasing amounts of NEFA induced a concentration-dependent increase in the proportion of LDL-. Moreover, a slightly increased NEFA content in LDL (15-50 mol NEFA/mol apoB) induced higher susceptibility to oxidation. These "in vitro" results concur with those observed in LDL obtained from athletes after exercise, i.e. a concentration of approximately 20 mol NEFA/mol apoB increased LDL oxidizability and LDL- proportion. We conclude that changes in the qualitative characteristics of LDL after exercise were unrelated to oxidative stress, but were related to the increase in LDL-associated NEFA content.