Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma

Blood. 2002 Jan 1;99(1):378-80. doi: 10.1182/blood.v99.1.378.


The hemoglobin scavenger receptor (HbSR/CD163) is an interleukin-6- and glucocorticoid-regulated macrophage/monocyte receptor for uptake of haptoglobin-hemoglobin complexes. Moreover, there are strong indications that HbSR serves an anti-inflammatory function. Immunoprecipitation and immunoblotting enabled identification of a soluble plasma form of HbSR (sHbSR) having an electrophoretic mobility equal to that of recombinant HbSR consisting of the extracellular domain (scavenger receptor cysteine-rich 1-9). A sandwich enzyme-linked immunosorbent assay was established and used to measure the sHbSR level in 130 healthy subjects (median, 1.87 mg/L; range, 0.73-4.69 mg/L). To evaluate the sHbSR levels in conditions with increased leukocyte stimulation and proliferation, 140 patients admitted to a hematological department were screened. Several patients, with a broad spectrum of diagnoses, had a level of sHbSR above the range of healthy persons. Patients with myelomonocytic leukemias and pneumonia/sepsis exhibited the highest levels (up to 67.3 mg/L). In conclusion, sHbSR is an abundant plasma protein potentially valuable in monitoring patients with infections and myelomonocytic leukemia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD*
  • Antigens, Differentiation, Myelomonocytic / blood*
  • Antigens, Differentiation, Myelomonocytic / genetics
  • CHO Cells
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gene Expression
  • Haptoglobins / metabolism*
  • Hemoglobins / metabolism*
  • Humans
  • Immunoblotting
  • Immunosorbent Techniques
  • Leukemia, Monocytic, Acute / blood
  • Leukemia, Myeloid, Acute / blood
  • Leukemia, Myelomonocytic, Acute / blood
  • Male
  • Pneumonia / blood
  • Receptors, Cell Surface / blood*
  • Receptors, Cell Surface / genetics
  • Recombinant Proteins
  • Reference Values
  • Sepsis / blood
  • Solubility
  • Transfection


  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • Haptoglobins
  • Hemoglobins
  • Receptors, Cell Surface
  • Recombinant Proteins