Abstract
Ceftazidime-susceptible and -resistant Pseudomonas aeruginosa strains were isolated from pulmonary specimens following a treatment with ceftazidime in a patient who developed a nosocomial pneumonia. The ceftazidime-susceptible and -resistant strains were clonally related and harbored a self-transferable approximately 155-kb plasmid. These isolates expressed two OXA-10-like oxacillinases, the narrow-spectrum OXA-35 and the expanded-spectrum OXA-19, respectively, differing by one amino acid substitution. This is the first example of in vivo selection of an extended-spectrum oxacillinase from a restricted-spectrum oxacillinase.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Carrier Proteins / genetics*
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Carrier Proteins / pharmacology*
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Ceftazidime / pharmacology*
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Cephalosporin Resistance / genetics*
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Cephalosporins / pharmacology*
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Conjugation, Genetic / genetics
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DNA, Bacterial / genetics
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Escherichia coli / genetics
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Humans
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Male
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Microbial Sensitivity Tests
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Middle Aged
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Molecular Sequence Data
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Plasmids / genetics
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Pseudomonas Infections / microbiology
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Pseudomonas aeruginosa / drug effects*
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beta-Lactamases / genetics*
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beta-Lactamases / pharmacology*
Substances
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Anti-Bacterial Agents
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Carrier Proteins
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Cephalosporins
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DNA, Bacterial
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Ceftazidime
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beta-Lactamases
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oxacillinase